Research article - Peer-reviewed, 2018
Identification of genes regulating traits targeted for domestication of field cress (Lepidium campestre) as a biennial and perennial oilseed crop
Gustafsson, Cecilia; Willforss, Jakob; Lopes-Pinto, Fernando; Ortiz, Rodomiro; Geleta, MulatuAbstract
Background: The changing climate and the desire to use renewable oil sources necessitate the development of new oilseed crops. Field cress (Lepidium campestre) is a species in the Brassicaceae family that has been targeted for domestication not only as an oilseed crop that produces seeds with a desirable industrial oil quality but also as a cover/catch crop that provides valuable ecosystem services. Lepidium is closely related to Arabidopsis and display significant proportions of syntenic regions in their genomes. Arabidopsis genes are among the most characterized genes in the plant kingdom and, hence, comparative genomics of Lepidium-Arabidopsis would facilitate the identification of Lepidium candidate genes regulating various desirable traits.Results: Homologues of 30 genes known to regulate vernalization, flowering time, pod shattering, oil content and quality in Arabidopsis were identified and partially characterized in Lepidium. Alignments of sequences representing field cress and two of its closely related perennial relatives: L. heterophyllum and L. hirtum revealed 243 polymorphic sites across the partial sequences of the 30 genes, of which 95 were within the predicted coding regions and 40 led to a change in amino acids of the target proteins. Within field cress, 34 polymorphic sites including nine nonsynonymous substitutions were identified. The phylogenetic analysis of the data revealed that field cress is more closely related to L. heterophyllum than to L. hirtum.Conclusions: There is significant variation within and among Lepidium species within partial sequences of the 30 genes known to regulate traits targeted in the present study. The variation within these genes are potentially useful to speed-up the process of domesticating field cress as future oil crop. The phylogenetic relationship between the Lepidium species revealed in this study does not only shed some light on Lepidium genome evolution but also provides important information to develop efficient schemes for interspecific hybridization between different Lepidium species as part of the domestication efforts.Keywords
Domestication; Field cress; Lepidium campestre; Pod shattering; VernalizationPublished in
BMC Genetics2018, volume: 19, article number: 36
Authors' information
Gustafsson, Cecilia (Hammenhag, Cecilia)
Swedish University of Agricultural Sciences, Department of Plant Breeding
Willforss, Jakob
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Lopes-Pinto, Fernando
Department of Animal Breeding and Genetics
Ortiz, Rodomiro (Ortiz Rios, Rodomiro Octavio)
Swedish University of Agricultural Sciences, Department of Plant Breeding
Geleta, Mulatu Dida (Dida, Mulatu Geleta)
Swedish University of Agricultural Sciences, Department of Plant Breeding
Lopes Pinto, Fernando A
Swedish University of Agricultural Sciences, Department of Animal Breeding and Genetics
Sustainable Development Goals
SDG13 Climate action
UKÄ Subject classification
Agricultural Science
Genetics
Publication Identifiers
DOI: https://doi.org/10.1186/s12863-018-0624-9
URI (permanent link to this page)
https://res.slu.se/id/publ/95345