Genome-wide expression analysis suggests a role for jasmonates in the resistance to blue mold in apple

Abstract

Blue mold, caused by the necrotrophic fungal pathogen Penicillium expansum, causes serious postharvest losses in apple, and threatens human health through production of the potent mycotoxin patulin. Recent studies indicate a quantitative control of resistance against this disease in apple cultivars. A whole genome apple microarray covering 60k transcripts was used to identify gene(s) that appear to be differentially regulated between resistant and susceptible cultivars in P. expansum-infected fruits. A number of potential candidates was encountered among defense- and oxidative stress-related genes, cell wall modification and lignification genes, and genes related to localization and transport. Induction of one cell wall-related gene and three genes involved in the 'down-stream' flavonoid biosynthesis pathway, demonstrates the fundamental role of the cell wall as an important barrier, and suggests that fruit flavonoids are involved in the resistance to blue mold. Moreover, exogenous application of the plant hormone methyl jasmonate (MeJA) reduced the symptoms resulting from inoculating apples with P. expansum. This is the first report linking MeJA and activation of cell wall and flavonoid pathway genes to resistance against blue mold in a study comparing different cultivars of domesticated apple. Our results provide an initial categorization of genes that are potentially involved in the resistance mechanism, and should be useful for developing tools for gene marker-assisted breeding of apple cultivars with an improved resistance to blue mold.

Keywords

Candidate gene; Cell wall; Flavonoid pathway; Jasmonic acid; Malus x domestica; Microarray

Published in

Plant Growth Regulation
2018, Volume: 85, number: 3, pages: 375-387

SLU Authors

• Ahmadi Afzadi, Masoud

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    
  • Graduate University of Advanced Technology (GUAT)

• Wera, Estelle

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    
  • Stockholm University

• Nybom, Hilde

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

Associated SLU-program

SLU Plant Protection Network


UKÄ Subject classification

Horticulture
Genetics and Breeding


Publication identifier

DOI: https://doi.org/10.1007/s10725-018-0388-2

Permanent link to this page (URI)

https://res.slu.se/id/publ/95471