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Research article1996Peer reviewed

Indocyanine green dye as a marker for measurement of plasma volume in goats

Rajaratne, AAJ; Hydbring, E; Dahlborn, K; Olsson, K


Evans blue dye disappears slowly from blood plasma and is not ideal for repeated measurements of plasma volume in the same goat because of increasing turbulence of the plasma. Therefore, indocyanine green (ICG) was evaluated as an alternative to Evans blue in goats. The plasma volume was 40 +/- 6 ml kg(-1) and 41 +/- 3 ml kg(-1) measured with ICG and Evans blue, respectively, in six non-pregnant, non-lactating goats. In three other goats plasma volume was measured during late lactation (7-8 months after parturition; milk production 1.0 +/- 0.6 l). It was 52 +/- 1 ml kg(-1) (ICG) and 51 +/- 5 ml kg(-1) (Evans blue), respectively. Thus, values of plasma volume measured by ICG corresponded well to these achieved with Evans blue. In a second series ICG was used repeatedly in the same goats to measure plasma volumes during late pregnancy and early lactation. A group of non-pregnant, non-lactating goats was used as control. Plasma volume was 39 +/- 3 ml kg(-1) (N = 7) and 44 +/- 8 ml kg(-1) (N = 6) at 23 +/- 7 days and 2.5 +/- 1 days before parturition, respectively. During early lactation it was 70 +/- 12 ml kg(-1) (23 +/- 5 days after parturition; milk production 2.4 +/- 0.5 l N = 7). No side effects were observed after several injections of ICG in any of the goats. In the control group, plasma volume was 40 +/- 7 ml kg(-1), which was the same value as that observed in the first series. We conclude that ICG is a good alternative to Evans blue for determination of plasma volume in goats.


indocyanine green; goat; plasma volume; pregnancy; lactation

Published in

Small Ruminant Research
1996, Volume: 19, number: 1, pages: 8683 Publisher: ELSEVIER SCIENCE BV

      SLU Authors

    • Sandberg, Eva

      • Department of Animal Physiology, Swedish University of Agricultural Sciences
      • Dahlborn, Kristina

        • Department of Animal Physiology, Swedish University of Agricultural Sciences
        • Olsson, Kerstin

          • Department of Animal Physiology, Swedish University of Agricultural Sciences

        UKÄ Subject classification

        Clinical Science
        Medical Bioscience

        Publication identifier


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