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Review article - Peer-reviewed, 2018

Practical guide to single layer centrifugation of stallion semen

Morrell, J. M.; Nunes, M. M.


The technique of single layer centrifugation through species-specific colloids has been gaining acceptance over the last few years as a practical method of improving the sperm quality of stallion semen for artificial insemination. This article explains the background to the technique and the different colloid formulations used, suggests some applications of the technique with particular reference to the equine breeding industry, describes the method in detail, and finally provides some guidance for problem solving. It is intended as a practical guide for those considering using the technique to improve sperm quality and pregnancy rates in equine artificial insemination and embryo transfer.


horse; sperm preparation; artificial insemination; colloid centrifugation; Androcoll-E; fertility

Published in

Equine Veterinary Education
2018, Volume: 30, number: 7, pages: 392-398
Publisher: WILEY

    UKÄ Subject classification

    Animal and Dairy Science

    Publication Identifiers


    Permanent link to this page (URI)