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Research article - Peer-reviewed, 1992

Syntheses of 14C‐Ochratoxin A and 14C‐Ochratoxin B and a Comparative Study of their Distribution in Rats Using Whole Body Autoradiography

BREITHOLTZEMANUELSSON, A; FUCHS, R; HULT, K; APPELGREN, LE

Abstract

Methods for preparation of labelled ochratoxin A and B are described. The method for preparation of labelled ochratoxin B involves the synthesis of the azide of ochratoxin-beta via the mixed anhydride and subsequent conjugation to labelled phenylalanine to yield C-14-ochratoxin B. The labelled ochratoxins were injected into male Wistar rats and after different survival times they were sacrificed and subjected to whole body autoradiography. The distribution pattern of ochratoxin A in the rat did not differ from that earlier registered for mouse. The previously known, high susceptibility of rats (and not mice) to ochratoxin A-induced cancer could thus not be explained by an accumulation of the toxin in specific cells or organs. The distribution patterns of ochratoxin A and B were almost congruent - the only apparent difference being a much longer retention of the labelled ochratoxin A in the blood compared to ochratoxin B, which was much faster excreted. When analyzing tissue extracts for labelled metabolites only the extracts from the rats injected with ochratoxin B were found to contain easily detectable concentrations, while no metabolites of ochratoxin A were seen.

Published in

Pharmacology & Toxicology
1992, Volume: 70, number: 4, pages: 255-261
Publisher: MUNKSGAARD INT PUBL LTD

      SLU Authors

    • Appelgren, Lars-Erik

      • Department of Pharmacology and Toxicology, Swedish University of Agricultural Sciences

    UKÄ Subject classification

    Pathobiology

    Publication identifier

    DOI: https://doi.org/10.1111/j.1600-0773.1992.tb00467.x

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/97431