- Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences
- University of Edinburgh
Impact of index hopping and bias towards the reference allele on accuracy of genotype calls from low-coverage sequencing
Ros-Freixedes, Roger; Battagin, Mara; Johnsson, Martin; Gorjanc, Gregor; Mileham, Alan J.; Rounsley, Steve D.; Hickey, John M.
BackgroundInherent sources of error and bias that affect the quality of sequence data include index hopping and bias towards the reference allele. The impact of these artefacts is likely greater for low-coverage data than for high-coverage data because low-coverage data has scant information and many standard tools for processing sequence data were designed for high-coverage data. With the proliferation of cost-effective low-coverage sequencing, there is a need to understand the impact of these errors and bias on resulting genotype calls from low-coverage sequencing.ResultsWe used a dataset of 26 pigs sequenced both at 2x with multiplexing and at 30x without multiplexing to show that index hopping and bias towards the reference allele due to alignment had little impact on genotype calls. However, pruning of alternative haplotypes supported by a number of reads below a predefined threshold, which is a default and desired step of some variant callers for removing potential sequencing errors in high-coverage data, introduced an unexpected bias towards the reference allele when applied to low-coverage sequence data. This bias reduced best-guess genotype concordance of low-coverage sequence data by 19.0 absolute percentage points.ConclusionsWe propose a simple pipeline to correct the preferential bias towards the reference allele that can occur during variant discovery and we recommend that users of low-coverage sequence data be wary of unexpected biases that may be produced by bioinformatic tools that were designed for high-coverage sequence data.
Genetics Selection Evolution
2018, Volume: 50, article number: 64
UKÄ Subject classification
Animal and Dairy Science
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