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Research article - Peer-reviewed, 2019

Perfluorononanoic acid (PFNA) alters lipid accumulation in bovine blastocysts after oocyte exposure during in vitro maturation

Hallberg, Ida; Kjellgren, Jacklin; Persson, Sara; Orn, Stefan; Sjunnesson, Ylva

Abstract

Perfluorononanoic acid (PFNA) is one of the perfluoroalkyl acids present in human tissues. In this study, effects on early embryo development after PFNA exposure were investigated using the bovine in vitro production system. Oocytes were exposed to PFNA during maturation in vitro (10 mu g mL(-1) and 0.1 mu g mL(-1)), and then fertilized and cultured in parallel with control groups. Developmental parameters (cleavage, blastocyst formation) were followed and embryo quality evaluated (stage, grade). Embryos developed after exposure to 0.1 mu g mL(-1) were stained to distinguish nuclei, active mitochondria and neutral lipids. 10 mu g mL(-1) of PFNA had a severe negative effect on blastocyst formation (OR: 0.27 p < 0.05), an effect not observed at 0.1 mu g mL(-1). However, lipid droplet distribution was significantly altered in embryos exposed to 0.1 mu g mL(-1), suggesting a disturbance of lipid metabolism after exposure to sublethal levels of PFNA during oocyte maturation in vitro.

Keywords

Bovine; IVP; PFNA; Lipid droplet; Image analysis; Developmental toxicology; Endocrine disruption

Published in

Reproductive Toxicology
2019, volume: 84, pages: 1-8

Authors' information

Kjellgren, Jacklin
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Swedish University of Agricultural Sciences, Department of Clinical Sciences

UKÄ Subject classification

Medical Bioscience
Clinical Science
Other Veterinary Science

Publication Identifiers

DOI: https://doi.org/10.1016/j.reprotox.2018.11.005

URI (permanent link to this page)

https://res.slu.se/id/publ/97652