Ma, Qian
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
Research article2019Peer reviewedOpen access
New fluorescently labeled auxins exhibit promising anti-auxin activity
Bieleszova, Kristyna; Parizkova, Barbora; Kubes, Martin; Husickova, Alexandra; Kubala, Martin; Ma, Qian; Sedlarova, Michaela; Robert, Stephanie; Dolezal, Karel; Strnad, Miroslav; Novak, Ondrej; Zukauskaite, Asta
Abstract
The plant hormone auxin is a key player in the regulation of plant growth and development. Despite numerous studies devoted to understanding its role in a wide spectrum of physiological processes, full appreciation of its function is linked to a comprehensive determination of its spatio-temporal distribution, which plays a crucial role in its mode of action. Conjugation of fluorescent tracers to plant hormones enables sensitive and specific visualization of their subcellular and tissue-specific localization and transport in planta, which represents a powerful tool for plant physiology. However, to date, only a few fluorescently labeled auxins have been developed. We report the synthesis of four novel fluorescently labeled derivatives of indole-3-acetic acid (IAA) in the form of a conjugate with a nitrobenzoxadiazole (NBD) fluorophore together with validation of their biological activity. These compounds, unlike other previously reported auxins fluorescently labeled at N1 position (nitrogen of the indole ring), do not possess auxin activity but rather show dose-dependent inhibition of auxininduced effects, such as primary root growth inhibition, root hair growth and the auxin reporter DR5::GUS expression. Moreover, the study demonstrates the importance of the character of the linker and optimal choice of the labeling site in the preparation of fluorescently labeled auxins as important variables influencing their biological activity and fluorescent properties.
Keywords
Anti-auxin; Arabidopsis; Biological activity; DR5::GUS; Fluorescent labeling; Indole-3-acetic acid (IAA)
Published in
New Biotechnology
2019, Volume: 48, pages: 44-52
Publisher: ELSEVIER SCIENCE BV
Robert, Stephanie
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
SLU Authors
UKÄ Subject classification
Biochemicals
Biochemistry and Molecular Biology
Publication identifier
DOI: https://doi.org/10.1016/j.nbt.2018.06.003
Permanent link to this page (URI)
https://res.slu.se/id/publ/97792