Chromatographic retention of epigallocatechin gallate on oligo-β-cyclodextrin coupled sepharose media investigated using NMR

Abstract

The retention of epigallocatechin gallate (EGCG) on oligo-beta-cyclodextrin (oligo-beta-CD) bonded agarose chromatographic media was investigated. NMR spectroscopy in solution showed that the EGCG immerses into the beta-CD cavity. The association constant calculated by NMR titration was used to estimate a retention factor which accurately reflected chromatographic behaviour. This correlation suggests that oligo-beta-CD forms inclusion complexes with EGCG via the same mechanism as monomeric beta-CD.

Keywords

column liquid chromatography; nuclear magnetic resonance; beta-cylcodextrin-bonded stationary phases; epigallocatechin gallate; agarose

Published in

Chromatographia
2006, volume: 64, number: 45659, pages: 45849

SLU Authors

• Sandström, Corine

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

Publication identifier

• DOI: https://doi.org/10.1365/s10337-006-0819-5

Permanent link to this page (URI)

https://res.slu.se/id/publ/9793