- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
Mushtaq, Rakhshanda; Shahzad, Khurram; Mansoor, Shahid; Shah, Zahid Hussain; Alsamadany, Hameed; Mujtaba, Tahir; Al-Zahrani, Yahya; Alzahrani, Hind A. S.; Ahmed, Zaheer; Bashir, Aftab
Cotton leaf curl virus (CLCuV) disease is one of the major limiting factors in cotton production, particularly in widely cultivated Gossypium hirsutum varieties that are susceptible to attack by this virus. Several approaches have been employed to explore putative resistance genes in another cotton species, G. arboreum. However, the exact mechanisms conferring disease resistance in cotton are still unknown. In the current study, we used various approaches to identify possible resistance genes against CLCuV infection. We report the identification and isolation of a set of genes involved in the resistance response to viral infestation. PCR products containing genomic DNA gave multiple amplifications with a single primer in most reactions, and 38 fragments were cloned from G. arboreum and G. hirsutum. The sequences of cloned fragments belonged to various pathway genes and uncharacterized proteins. However, five amplified fragments (RM1, RM6, RM8, RM12 and RM31) showed similarity with R genes. Maximum homology (94 %) was observed with G. raimondii toll/interleukin receptor-like protein. BLAST search showed the homology of all resistance gene analogues (RGAs) with more than one chromosome, and multiple hits were observed on each chromosome for each RGA. Expression analysis through RT-PCR identified variable expression levels of the different RGAs in all tested genotypes. The expression level of RGAs differed between symptomatic and asymptomatic plants, with the exception of RGA 395, whose expression level was the same in both diseased and healthy plants. Knowledge of the interaction of these genes with various cotton pathogens could be utilized to improve the resistance of susceptible G. hirsutum and other plant species.
Asymptomatic; CottonGen; ESTs; expression; motifs; symptomatic
2018, Volume: 10, number: 6, article number: ply067
Publisher: OXFORD UNIV PRESS
SLU Plant Protection Network