Khan Kayani, Waqas
- Department of Plant Breeding, Swedish University of Agricultural Sciences
Research article2019Peer reviewed
Ali, Huma; Khan, Mubarak Ali; Kayani, Waqas Khan; Dilshad, Erum; Rani, Rehana; Khan, Raham Sher
Ajuga bracteosa an important medicinal herb, is getting endangered worldwide due to destructive harvesting by pharmaceutical industries in its different habitats. It is in dire need for protection and demands conservation and sustainable utilization. In the present study, effects of a-naphthalene acetic acid (NAA) under different spectral lights were estimated on the growth, secondary metabolism and biosynthesis of phenolic acids in adventitious roots (AR) cultures of A. bracteosa. Among the different spectral lights, highest AR induction frequency (88%) and formation of biomass (72 g/L FW and 22 g/L DW) were recorded in explants incubated in the presence of 1.5 mg/L NAA under yellow light. Maximum production of poly phenols (TPC;44.2 mg) and flavonoids (TFC;2.51 mg) were recorded in the AR cultures grown in the presence of blue light. Further, highest total protein content of (401.6 mu g) was detected in the AR in response to normal white light. Blue spectral light induced maximum superoxide dismutase (SOD; 2.5 nM) and peroxidase activity (POD;0.85 nM) respectively, in AR cultures. Compared with other monochromatic lights, red light significantly enhanced the antioxidant potential of the AR cultures. Analysis through High performance liquid chromatography (HPLC-DAD) revealed significant variations in the levels of important phenolic acids such as gallic acid, catechin, rutin, caffeic acid, myricetin and apigenin in the AR samples treated with the lights of different spectra.
Ajuga; Spectral light; Adventitious roots; Total protein; Protease; Phenolic acids
Journal of Photochemistry and Photobiology B: Biology
2019, Volume: 193, pages: 109-117
Plant Biotechnology
DOI: https://doi.org/10.1016/j.jphotobiol.2019.02.010
https://res.slu.se/id/publ/98959