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Research article - Peer-reviewed, 2019

Infection Levels of the Microsporidium Larssoniella duplicati in Populations of the Invasive Bark Beetle Ips duplicatus: From Native to New Outbreak Areas

Zimova, Sona; Resnerova, Karolina; Vanicka, Hana; Horak, Jakub; Trombik, Jiri; Kacprzyk, Magdalena; Lindeloew, Ake; Duduman, Mihai-Leonard; Holusa, Jaroslav


The microsporidium Larssoniella duplicati (Weiser, Holua, Zika, 2006) is a specific pathogen of the bark beetle Ips duplicatus (C.R. Sahlberg, 1836), which is a serious pest of Norway spruce (Picea abies (L.) H. Karst) in Europe. From 2011 to 2016, infection levels of L. duplicati and other pathogens in I. duplicatus populations were assessed along a gradient, ranging from areas in the north, where the beetle is native, to areas in the south, where the beetle has only recently invaded. The 21 study sites ranged in altitude from 229 to 1009 m a.s.l. We found that pathogen infection levels in I. duplicatus populations decreased from the native areas in the north to the new areas of beetle expansion in the south. We also found that pathogen level increased with altitude. The L. duplicati infection levels were not associated with the infection levels of other beetle natural enemies. The infection level decreased with the length of time of beetle establishment in an area. The infection level increased with the number of beetles trapped and dissected at a site.


Ips duplicatus; pathogen; vector; infection level; invasion; latitude

Published in

2019, volume: 10, number: 2, article number: 131
Publisher: MDPI

Authors' information

Zimova, Sona
Czech University of Life Sciences Prague
Resnerova, Karolina
Czech University of Life Sciences Prague
Vanicka, Hana
Czech University of Life Sciences Prague
Horak, Jakub
Czech University of Life Sciences Prague
Trombik, Jiri
Czech University of Life Sciences Prague
Kacprzyk, Magdalena
Agricultural University Krakow
Swedish University of Agricultural Sciences, Department of Ecology
Duduman, Mihai-Leonard
Lucian Blaga University of Sibiu
Holusa, Jaroslav
Czech University of Life Sciences Prague

UKÄ Subject classification

Forest Science

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