Backbone assignment of the MALT1 paracaspase by solution NMR

Abstract

Mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1) is a unique paracaspase protein whose protease activity mediates oncogenic NF-kappa B signalling in activated B cell-like diffuse large B cell lymphomas (ABC-DLBCLs). ABC-DLBCLs are aggressive lymphomas with high resistance to current chemotherapies. Low survival rate among patients emphasizes the urgent need for alternative treatment options. The characterization of the MALT1 will be an essential tool for developing new target-directed drugs against MALT1 dependent disorders. As the first step in the atomic-level NMR studies of the system, here we report, the N-15/C-13/H-1 backbone assignment of the apo form of the MALT1 paracaspase region together with the third immunoglobulin-like (Ig3) domain, 44 kDa, by high resolution NMR. In addition, the non-uniform sampling (NUS) based targeted acquisition procedure is evaluated as a mean of decreasing acquisition and analysis time for larger proteins.

Published in

PLoS ONE
2016, Volume: 11, number: 1, article number: e0146496
Publisher: PUBLIC LIBRARY SCIENCE

UKÄ Subject classification

Medical Biotechnology


Publication identifier

DOI: https://doi.org/10.1371/journal.pone.0146496

Permanent link to this page (URI)

https://res.slu.se/id/publ/99457