Abstract

A polymerase chain reaction (PCR) procedure was developed for analysis of viable Salmonella spp. in feed samples and scrapings from feed mills. The objective was to develop a cost-effective, user-friendly method that could be performed within ordinary working hours. A single pre-enrichment was enough for most feed raw materials. However, scrapings and pellets required additional pre-enrichment. The results indicate that inhibition of the PCR reaction was correlated with a pH value below 7.0 in the first pre-enrichment. Results of the PCR procedure were in agreement with the established culture-based Nordic Committee on Food Analysis method in spiked samples. The developed PCR procedure can be used as a routine analysis method for feed samples and samples from a Salmonella feed control program based on hazard analysis critical control point principles.

Published in

Journal of Rapid Methods and Automation in Microbiology
2005, Volume: 13, number: 2, pages: 96-110

UKÄ Subject classification

Pathobiology


Publication identifier

DOI: https://doi.org/10.1111/j.1745-4581.2005.00012.x

Permanent link to this page (URI)

https://res.slu.se/id/publ/99675