Research article - Peer-reviewed, 2018
Presynaptic Biogenesis Requires Axonal Transport of Lysosome-Related Vesicles
Vukoja, Anela; Hohensee, Svea; Rosa, Stefanie; Lipowsky, Reinhard; Sigrist, Stephan J.; Haucke, Volker; Rey, Ulises; Petzoldt, Astrid G.; Ott, Christoph; Vollweiter, Dennis; Quentin, Christine; Puchkov, Dymtro; Reynolds, Eric; Lehmann, MartinAbstract
Nervous system function relies on the polarized architecture of neurons, established by directional transport of pre-and postsynaptic cargoes. While delivery of postsynaptic components depends on the secretory pathway, the identity of the membrane compartment(s) supplying presynaptic active zone (AZ) and synaptic vesicle (SV) proteins is unclear. Live imaging in Drosophila larvae and mouse hippocampal neurons provides evidence that presynaptic biogenesis depends on axonal co-transport of SV and AZ proteins in presynaptic lysosome-related vesicles (PLVs). Loss of the lysosomal kinesin adaptor Arl8 results in the accumulation of SV- and AZ-protein-containing vesicles in neuronal cell bodies and a corresponding depletion of SV and AZ components from presynaptic sites, leading to impaired neurotransmission. Conversely, presynaptic function is facilitated upon overexpression of Arl8. Our data reveal an unexpected function for a lysosome-related organelle as an important building block for presynaptic biogenesis.Published in
Neuron2018, volume: 99, number: 6, pages: 1216-1232
Authors' information
Vukoja, Anela
Leibniz-Forschungsinstitut für Molekulare Pharmakologie
Rey, Ulises
Free University of Berlin
Petzoldt, Astrid G.
Free University of Berlin
Ott, Christoph
Leibniz-Forschungsinstitut für Molekulare Pharmakologie
Vollweiter, Dennis
Leibniz-Forschungsinstitut für Molekulare Pharmakologie
Quentin, Christine
Free University of Berlin
Puchkov, Dymtro
Leibniz-Forschungsinstitut für Molekulare Pharmakologie
Reynolds, Eric
Free University of Berlin
Lehmann, Martin
Leibniz-Forschungsinstitut für Molekulare Pharmakologie
Hohensee, Svea
Leibniz-Forschungsinstitut für Molekulare Pharmakologie
Free University of Berlin
Leibniz-Forschungsinstitut für Molekulare Pharmakologie
Lipowsky, Reinhard
Max Planck Society
Sigrist, Stephan J.
Free University of Berlin
Haucke, Volker
Humboldt University of Berlin
UKÄ Subject classification
Neurosciences
Publication Identifiers
DOI: https://doi.org/10.1016/j.neuron.2018.08.004
URI (permanent link to this page)
https://res.slu.se/id/publ/99694