Kalyandurg, Pruthvi Balachandra
- Department of Plant Biology, Swedish University of Agricultural Sciences
Research article2019Peer reviewedOpen access
Efficient RNA silencing suppression activity of Potato Mop-Top Virus 8K protein is driven by variability and positive selection
Kalyandurg, Pruthvi B.; Tahmasebi, Aminallah; Vetukuri, Ramesh R.; Kushwaha, Sandeep K.; Lezzhov, Alexander A.; Solovyev, Andrey G.; Grenville-Briggs, Laura J.; Savenkov, Eugene I.
Abstract
Previously, we investigated the evolution of Potato mop-top virus (PMTV) ORFs. Results indicate that positive selection acts exclusively on an ORF encoding the 8K protein, a weak viral suppressor of RNA silencing (VSR). However, how the extraordinary variability contributes to 8K-mediated RNA silencing suppression remains unknown. Here, we characterized the RNA silencing suppression activity of the 8K protein from seven diverse isolates. We show that 8K encoded by isolate P1 exhibits stronger RNA silencing suppression activity than the 8K protein from six other isolates. Mutational analyses revealed that Ser-50 is critical for these differences. By comparing small RNA profiles we found a lower abundance of siRNAs with U residue at the 5'-terminus after expression of the P1 8K compared to expression of 8K from isolate P125, an isolate with weak VSR activity. These results provide new clues as to the role of positive selection in shaping activities of VSRs.
Keywords
Natural variation; Next generation sequencing; Plant viruses; RNA silencing; siRNA; Viral suppressor of RNA silencing
Published in
Virology
2019, volume: 535, pages: 111-121
SLU Authors
Tahmasebi, Aminallah
- Department of Plant Biology, Swedish University of Agricultural Sciences
- Shiraz University
- Department of Plant Biology, Swedish University of Agricultural Sciences
Vetukuri, Ramesh
- Department of Plant Protection Biology, Swedish University of Agricultural Sciences
- Department of Plant Protection Biology, Swedish University of Agricultural Sciences
Kushwaha, Sandeep Kumar
- Department of Plant Breeding, Swedish University of Agricultural Sciences
- Department of Plant Breeding, Swedish University of Agricultural Sciences
Grenville-Briggs Didymus, Laura
- Department of Plant Protection Biology, Swedish University of Agricultural Sciences
- Department of Plant Protection Biology, Swedish University of Agricultural Sciences
Savenkov, Eugene
- Department of Plant Biology, Swedish University of Agricultural Sciences
- Department of Plant Biology, Swedish University of Agricultural Sciences
Associated SLU-program
SLU Plant Protection Network
UKÄ Subject classification
Agricultural Science
Publication identifier
- DOI: https://doi.org/10.1016/j.virol.2019.06.018
Permanent link to this page (URI)
https://res.slu.se/id/publ/101175