Diversity and occurrence of chickpea chlorotic dwarf virus on legumes from Iran

Abstract

Chickpea chlorotic dwarf virus (CpCDV; genus Mastrevirus, family Geminiviridae) is one of the most important legume-infecting viruses with a wide host range and geographic distribution in Africa and Asia. In Iran, CpCDV is common in chickpea (Cicer arietinum), but there is limited information about diversity and infections in plants of other legume species. In the current study, a total of 1671 leaf samples from different pulse crops with symptoms were collected in nine provinces of Iran, and the CpCDV infection status was tested by PCR and/or rolling circle amplification (RCA), resulting in the detection of CpCDV in samples of chickpea, lentil (Lens culinaris) and faba bean (Vicia faba) from different regions. Sequence analysis of complete genomes of 18 isolates recovered by digestion of RCA products revealed infection with isolates of the strains CpCDV-A and CpCDV-F in chickpea, lentil and faba bean. Phylogenetic analysis showed that the Iranian isolates of CpCDV were closely related to previously sequenced isolates of CpCDV-A and CpCDV-F. To the authors' knowledge, this is the first report of CpCDV-F in Iran. Using agroinoculation with infectious clones for one isolate each of CpCDV-A and CpCDV-F, infectivity was confirmed in both faba bean and chickpea, with plants developing leaf curling and/or yellowing. Both infectious clones also successfully infected Nicotiana benthamiana resulting in mild yellowing and intensive leaf curling for CpCDV-A, and dark-green mosaic, dwarfing and mild leaf curling for CpCDV-F.

Keywords

agroinoculation; CpCDV strain F; genetic diversity; infectious clone; mastrevirus; pulse crops

Published in

Plant Pathology
2020, volume: 69, number: 1, pages: 139-148
Publisher: WILEY

SLU Authors

• Kvarnheden, Anders

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

Associated SLU-program

SLU Plant Protection Network

Global goals (SDG)

SDG2 Zero hunger

UKÄ Subject classification

Agricultural Science

Publication identifier

• DOI: https://doi.org/10.1111/ppa.13098

Permanent link to this page (URI)

https://res.slu.se/id/publ/102262