Effects of dietary supplementation of lignocellulose-derived cello-oligosaccharides on growth performance, antioxidant capacity, immune response, and intestinal microbiota in rainbow trout (Oncorhynchus mykiss)

Abstract

This study evaluated the prebiotic potential of cello-oligosaccharides (COS) produced from birch (Betula pen-dula), an under-utilised lignocellulosic source from the forestry industry, on growth performance, mucosal im-munity, gut microbiota composition, and antioxidant capacity of juvenile rainbow trout (Oncorhynchus mykiss). In a 45-day trial, the fish were fed with diets containing 0%, 0.1%, 0.5% and 1.5% COS, while a diet containing fructo-oligosaccharides (0.5% FOS) was used as a positive control. Fish fed with the 0.5% and 1.5% COS diets showed significantly (P < 0.05) higher abundance of Ruminococcaceae, Bacillaceae and Lactobacillaceae, in the faecal microbiota. The COS diets also induced higher antioxidant capacity in the gut and serum, but there were no treatment effects (P > 0.05) on growth of rainbow trout. Gene expression analysis of the intestine showed significant elevation (P < 0.05) in expression of complement (c3 and c-type lectin) and receptor (tlr2) genes of the innate immune system in COS-fed fish. However, for cytokine and adaptive immune genes, no significant dif-ferences (P > 0.05) in gene transcripts were observed between the COS/FOS diets with the control diet. These results suggest that dietary cello-oligosaccharides can be a useful feed supplement for rainbow trout, which can modulate intestinal microbial communities, innate immune response and antioxidant capacity of the host.

Keywords

Cello-oligosaccharides; Rainbow trout; Microbiota; Forest waste; Gut immunity; Oxidative stress

Published in

Aquaculture
2024, volume: 578, article number: 740002

SLU Authors

• Singh, Aprajita

  • Department of Applied Animal Science and Welfare, Swedish University of Agricultural Sciences
    

• Vidakovic, Aleksandar

  • Department of Applied Animal Science and Welfare, Swedish University of Agricultural Sciences
    

• Hjertner, Bernt

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Dicksved, Johan

  • Department of Applied Animal Science and Welfare, Swedish University of Agricultural Sciences
    

• Baruah, Kartik

  • Department of Applied Animal Science and Welfare, Swedish University of Agricultural Sciences
    

• Lundh, Torbjörn

  • Department of Applied Animal Science and Welfare, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Fish and Aquacultural Science

Publication identifier

• DOI: https://doi.org/10.1016/j.aquaculture.2023.740002

Permanent link to this page (URI)

https://res.slu.se/id/publ/123580