Chemically Induced Extracellular Ice Nucleation Reduces Intracellular Ice Formation Enabling 2D and 3D Cellular Cryopreservation

Abstract

3D cell assemblies such as spheroids reproduce the in vivo state more accurately than traditional 2D cell monolayers and are emerging as tools to reduce or replace animal testing. Current cryopreservation methods are not optimized for complex cell models, hence they are not easily banked and not as widely used as 2D models. Here we use soluble ice nucleating polysaccharides to nucleate extracellular ice and dramatically improve spheroid cryopreservation outcomes. This protects the cells beyond using DMSO alone, and with the major advantage that the nucleators function extracellularly and hence do not need to permeate the 3D cell models. Critical comparison of suspension, 2D and 3D cryopreservation outcomes demonstrated that warm-temperature ice nucleation reduces the formation of (fatal) intracellular ice, and in the case of 2/3D models this reduces propagation of ice between adjacent cells. This demonstrates that extracellular chemical nucleators could revolutionize the banking and deployment of advanced cell models.

Keywords

Cryopreservation; 3D cell assemblies; ice nucleation; polysaccharides; chemical control of ice formation

Published in

JACS Au
2023, volume: 3, number: 5, pages: 1314-1320

SLU Authors

• Griffiths, Christopher

  • Department of Aquatic Resources (SLU Aqua), Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Cell Biology

Publication identifier

• DOI: https://doi.org/10.1021/jacsau.3c00056

Permanent link to this page (URI)

https://res.slu.se/id/publ/123850