Elevated levels of IL-12/IL-23p40 in Nova Scotia Duck Tolling Retrievers with autoimmune disease and lymphoma

Abstract

The Nova Scotia Duck Tolling Retriever (NSDTR) is predisposed to immune mediated rheumatic disease (IMRD), steroid-responsive meningitis-arteritis (SRMA) and certain forms of cancer. Cytokines are the main regulators of the immune system. Interleukin 2 is a cytokine involved in activation of T regulatory cells, playing a role in central tolerance and tumor immunity. Interleukin 12 and interleukin 23 share the same subunit, p40, and are both pro-inflammatory cytokines. The aim of this study was to compare levels of IL-2 in healthy NSDTRs to those with cancer or autoimmune disease and to compare levels of IL-12/IL-23p40 in healthy NSDTRs and beagles versus NSDTRs with cancer or autoimmune disease. 62 dogs were included in the analysis of IL-12/IL-23p40; healthy NSDTRs (n = 16), healthy beagles (n = 16), NSDTRs autoimmune (n = 18) and NDSTRs lymphoma/mastocytoma (n = 12) and 68 dogs for IL-2; healthy (n = 20), autoimmune (n = 36) and lymphoma/mastocytoma/adenocarcinoma (n = 12). NSDTRs with autoimmune disease had higher levels of IL-12/IL-23p40 compared to healthy dogs (p = 0.008). NSDTRs with lymphoma also had higher levels of IL-12/IL-23p40 compared to healthy NSDTRs (p = 0.002). There was no difference in levels of IL-2 between healthy and diseased NSDTR. Statistical analysis was performed using Bonferroni corrections for multiple testing. These findings can contribute to the knowledge of autoimmune disease and cancer in dogs.

Keywords

Autoimmune; Immune-mediated rheumatic disease; Steroid-responsive meningitis-arteritis; IMRD; SRMA; NSDTR; Canine tumors; Cytokines

Published in

Scientific Reports
2024, volume: 14, number: 1, article number: 11624
Publisher: NATURE PORTFOLIO

SLU Authors

• Nilsson, Malin

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Saellström, Sara

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Johansson, Siri

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Andersson, Göran

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Hamlin, Helene

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Rönnberg, Henrik

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Pathobiology
Clinical Science

Publication identifier

• DOI: https://doi.org/10.1038/s41598-024-62265-y

Permanent link to this page (URI)

https://res.slu.se/id/publ/131120