Comparing plant litter molecular diversity assessed from proximate analysis and 13C NMR spectroscopy

Abstract

Accurate representation of the chemical diversity of litter in ecosystem-scale models is critical for improving predictions of decomposition rates and stabilization of plant material into soil organic matter. In this contribution, we conducted a systematic review to evaluate how conventional characterization of plant litter quality using proximate analysis compares with molecular-scale characterization using 13 C NMR spectroscopy. Using a molecular mixing model, we converted chemical shift regions from NMR into fractions of carbon (C) in five organic compound classes that are major constituents of plant material: carbohydrates, proteins, lignins, lipids, and carbonylic compounds. We found positive correlations between the acid soluble fraction and carbohydrates, and between the acid insoluble fraction and lignins. However, the acid-soluble fraction underestimated carbohydrates, and the acid insoluble fraction overestimated lignins by 243%. We identified two sources of uncertainties: i) disparities between litter chemical composition based on hydrolysability and actual chemical composition obtained from NMR and ii) conversion factors to translate proximate fractions into organic constituents. Both uncertainties are critical, potentially leading to misinterpretations of decay rates in litter decomposition models. Consequently, we recommend including explicit substrate chemistry data in the next generation of litter decomposition models.

Published in

Soil Biology and Biochemistry
2024, volume: 197, article number: 109517
Publisher: PERGAMON-ELSEVIER SCIENCE LTD

SLU Authors

• Lindahl, Björn

  • Department of Soil and Environment, Swedish University of Agricultural Sciences
    

Associated SLU-program

SLUsystematic

UKÄ Subject classification

Soil Science

Publication identifier

• DOI: https://doi.org/10.1016/j.soilbio.2024.109517

Permanent link to this page (URI)

https://res.slu.se/id/publ/131528