Cytotoxicity and Oxidative Stress Induced by Technology-Critical Elements versus Traditional Metal Contaminants: An In Vitro Bioassay Study

Abstract

Technology-critical elements (TCEs), essential in emerging technologies, are increasingly finding their way into our environment, raising concerns about their sparsely studied behavior and toxicity. To contribute insights into the toxicological aspects, we employed in vitro bioassays to investigate the possible cytotoxic effects in four representative cell lines (AR-EcoScreen GR-KO-M1, DR-EcoScreen, MCF7AREc32, VM7Luc4E2) and the potential to induce oxidative stress via the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway for a number of these elements. Nine TCEs, three rare-earth elements (REEs: Gd, Nd, Yb) and six less-studied TCEs (LSTCEs: Ga, Ge, In, Ta, Te, Tl), were selected for this study, along with three well-studied traditional metal contaminants (TMCs: As, Cd, Pb) for comparison. Among the 12 studied elements, nine showed signs of inducing cytotoxicity: As, Cd, Ga, Nd, and Te in three out of the four studied cell lines and Gd, Ta, Tl, and Yb in one to two cell lines. Tellurium repeatedly exhibited the highest potency. The TCEs Ga and In, similar to As and Cd, also demonstrated the potential to induce oxidative stress. The results of this study suggest that some TCEs may potentially cause adverse health effects similar to As and Cd, thus prompting further investigations.

Keywords

emerging contaminants; metals; health risks; toxicity; reportergenes

Published in

Environmental Science and Technology
2025
Publisher: AMER CHEMICAL SOC

SLU Authors

• Qvarforth, Anna

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    
  • Linnaeus University

• von Ehr, Michelle

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Mandava, Geeta

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Lundqvist, Johan

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Environmental Sciences

Publication identifier

• DOI: https://doi.org/10.1021/acs.est.4c09710

Permanent link to this page (URI)

https://res.slu.se/id/publ/140192