Metabolomic evaluation of pulsed electric field-induced stress on potato tissue

Abstract

Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.

Published in

Planta
2009, volume: 230, number: 3, pages: 469-479

SLU Authors

• Lundgren, Krister

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

• Moritz, Thomas

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Forest Science

Publication identifier

• DOI: https://doi.org/10.1007/s00425-009-0950-2

Permanent link to this page (URI)

https://res.slu.se/id/publ/26208