A von Willebrand factor-binding protein from Staphylococcus lugdunensis

Abstract

In the present study, a phage display library covering the genome of Staphylococcus lugdunensis, was affinity-selected against von Willebrand factor (vWf). This led to the identification of a gene, vwbl, encoding a Putative cell surface protein of 2060 amino acids, denoted vWbl. The deduced protein has an overall organisation typical of staphylococcal cell Surface proteins, with an N-terminal signal peptide, and a C-terminal cell wall sorting signal. The vWf-binding part is located in repetitive domains and antibodies against vWbl or vWf can inhibit the binding. Southern blot analysis showed that vwbl was present in the 12 S. lugdunensis strains tested. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved

Published in

FEMS Microbiology Letters
2004, volume: 234, number: 1, pages: 155-161
Publisher: ELSEVIER SCIENCE BV

SLU Authors

• Bjerketorp, Joakim

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Frykberg, Lars

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Guss, Bengt

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Animal and Dairy Science
Veterinary Science

Publication identifier

• DOI: https://doi.org/10.1016/j.femsle.2004.03.024

Permanent link to this page (URI)

https://res.slu.se/id/publ/2816