Sorting a Staphylococcus aureus phage display library against ex vivo biomaterial

Abstract

A phage display library made from Staphylococcus aureus DNA was sorted against a central venous catheter (CVC) that had been removed from a patient 2 days after insertion. After the first panning, approximately 50% of the clones encoded proteins known to interact with mammalian proteins. After the second and third pannings, fibrinogen-binding and beta(2)-glycoprotein I (beta(2)-GPI)-binding phage particles were clearly dominating. Proteins adsorbed to different CVCs were investigated using specific antibodies. Among the proteins probed for, fibrinogen was most abundant, but, interestingly, beta(2)-GPI was also detected on all tested CVCs

Keywords

Staphylococcus aureus; phage display; biomaterial; receptin; beta 2-glycoprotein I

Published in

Journal of Medical Microbiology
2004, volume: 53, number: 10, pages: 945-951
Publisher: SOC GENERAL MICROBIOLOGY

SLU Authors

• Bjerketorp, Joakim

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Frykberg, Lars

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Jacobsson, Karin

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

Publication identifier

• DOI: https://doi.org/10.1099/jmm.0.45638-0

Permanent link to this page (URI)

https://res.slu.se/id/publ/3036