Vagiri, Michael
- Department of Plant Breeding, Swedish University of Agricultural Sciences
Research article2012Peer reviewed
An Optimized Method for Analysis of Phenolic Compounds in Buds, Leaves, and Fruits of Black Currant (Ribes nigrum L.)
Vagiri, Michael; Ekholm, Anders; Andersson, Staffan; Johansson, Eva; Rumpunen, Kimmo
Abstract
Although the fruits are the economic driver for the black currant industry, the buds and leaves are excellent sources of beneficial phenolic compounds that may contribute to the future value of the crop. In this study, extraction of phenolic compounds for different parts of the black currant plant was optimized, and an efficient method for their separation by HPLC was developed. This allowed the simultaneous quantification of a range of hydroxycinnamic acids, flavan-3-ols, flavonols, and anthocyanins by DAD following their identification by HPLC-ESI-MSn. A total of 23 compounds were detected in the buds, 22 of which were found in fruit and leaves. To the best of our knowledge, this is the first report of flavonol glycosides of quercetin, myricetin, isorhamnetin, and kaempferol along with hydroxycinnamic acids such as neo-chlorogenic acid and chlorogenic acid in the buds. Additionally, we provide the first evidence of kaempferol-3-O-rutinoside in black currant leaves. This approach offers avenues for superior combined compositional identification and cultivar selection targeted at the generation of polyphenol-rich products derived from the whole crop and not just the fruit.
Keywords
anthocyanins; ascorbic acid; bioactive compounds; extraction; flavonol; functional food; HPLC; polyphenols
Published in
Journal of Agricultural and Food Chemistry
2012, volume: 60, number: 42, pages: 10501-10510
Publisher: AMER CHEMICAL SOC
SLU Authors
Ekholm, Anders
- Department of Plant Breeding, Swedish University of Agricultural Sciences
- Department of Plant Breeding, Swedish University of Agricultural Sciences
Andersson, Staffan
- Department of Plant Breeding, Swedish University of Agricultural Sciences
- Department of Plant Breeding, Swedish University of Agricultural Sciences
Johansson, Eva
- Department of Biosystems and Technology, Swedish University of Agricultural Sciences
- Department of Biosystems and Technology, Swedish University of Agricultural Sciences
Rumpunen, Kimmo
- Department of Plant Breeding, Swedish University of Agricultural Sciences
- Department of Plant Breeding, Swedish University of Agricultural Sciences
UKÄ Subject classification
Food Science
Horticulture
Publication identifier
- DOI: https://doi.org/10.1021/jf303398z
Permanent link to this page (URI)
https://res.slu.se/id/publ/41824