Species specific primers for identification of Alternaria solani, in combination with analysis of the F129L substitution associates with loss of sensitivity toward strobilurins

Abstract

A species specific forward primer was designed in order to identify the phytopathogenic fungus Alternaria solani, which causes early blight on potato. The reverse primer came from another survey of the American population of A. solani. The combination of these two primers can be used both as molecular identification of A. solani as well as sending the PCR product for sequencing in search for the F129L substitution associated with loss of sensitivity toward fungicides based on strobilurins. The diagnostic method has been successfully used when identifying the causal agent of lesions on potato leaflets during large field assays. (C) 2012 Elsevier Ltd. All rights reserved.

Keywords

Molecular diagnostics; PCR; Species-specific primers; Fungicide resistance; Alternaria solani

Published in

Crop Protection
2012, volume: 38, pages: 72-73
Publisher: ELSEVIER SCI LTD

SLU Authors

• Edin, Eva

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

Associated SLU-program

AMR: Fungus

UKÄ Subject classification

Agricultural Science

Publication identifier

• DOI: https://doi.org/10.1016/j.cropro.2012.03.021

Permanent link to this page (URI)

https://res.slu.se/id/publ/45305