Electrochemical impedance spectroscopy in label-free biosensor applications: multivariate data analysis for an objective interpretation

Abstract

Electrochemical impedance spectroscopy plays an important role in biosensor science thanks to the possibility of finding specific information from processes with different kinetics at a chosen electrode potential in one experiment. In this paper we briefly discuss label-free impedimetric biosensors described in the literature. A novel method for neutral interpretation of impedance data is presented that includes complex number chemometrics. Three examples are given based on impedance measurements on synthetic biomembranes, in this case a lipid monolayer deposited on a mercury electrode. The interaction of various compounds with the monomolecular lipid layer is illustrated with the following: (1) different concentrations of magainin (Geladi et al. in Proc. Int. Fed. Med. Biomed. Eng. 9:219-220, 2005); (2) different derivatives of gramicidin A (Lindholm-Sethson et al. in Langmuir 24:5029-5032, 2007), and (3) an antimicrobial peptide (Ringstad et al. in Langmuir 24:208-216, 2008).

Keywords

Impedance; Biosensor; Complex number chemometrics

Published in

Analytical and Bioanalytical Chemistry
2010, volume: 398, number: 6, pages: 2341-2349
Publisher: SPRINGER HEIDELBERG

SLU Authors

• Nyström, Josefina

  • Umeå University

• Geladi, Paul

  • Department of Forest Biomaterials and Technology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Biochemistry and Molecular Biology
Analytical Chemistry

Publication identifier

• DOI: https://doi.org/10.1007/s00216-010-4027-7

Permanent link to this page (URI)

https://res.slu.se/id/publ/47806