Plant secretome proteomics

Abstract

The plant secretome refers to the set of proteins secreted out of the plant cell into the surrounding extracellular space commonly referred to as the apoplast. Secreted proteins maintain cell structure and acts in signaling and are crucial for stress responses where they can interact with pathogen effectors and control the extracellular environment. Typically, secreted proteins contain an N-terminal signal peptide and are directed through the endoplasmic reticulum/Golgi pathway. However, in plants many proteins found in the secretome lack such a signature and might follow alternative ways of secretion. This review covers techniques to isolate plant secretomes and how to identify and quantify their constituent proteins. Furthermore, bioinformatical tools to predict secretion signals and define the putative secretome are presented. Findings from proteomic studies and important protein families of plant secretomes, such as proteases and hydrolases, are highlighted.

Keywords

apoplast; mass spectrometry; plant; proteomics; secretome

Published in

Frontiers in Plant Science
2013, volume: 4
Publisher: FRONTIERS RESEARCH FOUNDATION

SLU Authors

• Alexandersson, Erik

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

• Ali, Ashfaq

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

• Resjö, Svante

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

• Andreasson, Erik

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Biochemistry and Molecular Biology

Publication identifier

• DOI: https://doi.org/10.3389/fpls.2013.00009

Permanent link to this page (URI)

https://res.slu.se/id/publ/56140