Transcriptome Analysis of High-Temperature Stress in Developing Barley Caryopses: Early Stress Responses and Effects on Storage Compound Biosynthesis

Mangelsen, Elke; Kilian, Joachim; Harter, Klaus; Jansson, Christer; Wanke, Dierk; Sundberg, Eva

doi:10.1093/mp/ssq058

Research article2011Peer reviewedOpen access

Transcriptome Analysis of High-Temperature Stress in Developing Barley Caryopses: Early Stress Responses and Effects on Storage Compound Biosynthesis

Mangelsen, Elke; Kilian, Joachim; Harter, Klaus; Jansson, Christer; Wanke, Dierk; Sundberg, Eva

Abstract

High-temperature stress, like any abiotic stress, impairs the physiology and development of plants, including the stages of seed setting and ripening. We used the Affymetrix 22K Barley1 GeneChip microarray to investigate the response of developing barley (Hordeum vulgare) seeds, termed caryopses, after 0.5, 3, and 6 h of heat stress exposure; 958 induced and 1122 repressed genes exhibited spatial and temporal expression patterns that provide a detailed insight into the caryopses' early heat stress responses. Down-regulation of genes related to storage compound biosynthesis and cell growth provides evidence for a rapid impairment of the caryopsis' development. Increased levels of sugars and amino acids were indicative for both production of compatible solutes and feedback-induced accumulation of substrates for storage compound biosynthesis. Metadata analysis identified embryo and endosperm as primary locations of heat stress responses, indicating a strong impact of short-term heat stress on central developmental functions of the caryopsis. A comparison with heat stress responses in Arabidopsis shoots and drought stress responses in barley caryopses identified both conserved and presumably heat- and caryopsis-specific stress-responsive genes. Summarized, our data provide an important basis for further investigation of gene functions in order to aid an improved heat tolerance and reduced losses of yield in barley as a model for cereal crops.

Keywords

Abiotic; environmental stress; gene expression; transcriptome analysis; seed biology; barley; caryopsis; crops; heat shock

Published in

Molecular Plant
2011, volume: 4, number: 1, pages: 97-115
Publisher: OXFORD UNIV PRESS

SLU Authors

Mangelsen, Elke
- Department of Plant Biology, Swedish University of Agricultural Sciences
Sundberg, Eva
- Department of Plant Biology, Swedish University of Agricultural Sciences

UKÄ Subject classification

Agricultural Science
Food Science

Publication identifier

DOI: https://doi.org/10.1093/mp/ssq058

Permanent link to this page (URI)

https://res.slu.se/id/publ/57376