Detection of Chalara fraxinea from tissue of Fraxinus excelsior using species-specific ITS primers

Abstract

P>Chalara fraxinea (teleomorph: Hymenoscyphus albidus) is known as a serious pathogen of Fraxinus excelsior, causing massive dieback of trees in Europe. The fungus is able to cause latent infections, and has been previously detected as an endophyte in asymptomatic tissues. Chalara fraxinea is a slow grower in culture, and is thus likely to be overgrown by faster growing fungi whenever pure culture isolations are being attempted. This study reports species-specific ITS primers allowing fast and reliable detection of the pathogen directly from infected tissues of F. excelsior.

Published in

Forest Pathology
2010, volume: 40, number: 2, pages: 111-115
Publisher: WILEY-BLACKWELL PUBLISHING, INC

SLU Authors

• Bengtsson, Stina

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Vasaitis, Rimvydas

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Ihrmark, Katarina

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Barklund, Pia

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Stenlid, Jan

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Forest Science

Publication identifier

• DOI: https://doi.org/10.1111/j.1439-0329.2009.00614.x

Permanent link to this page (URI)

https://res.slu.se/id/publ/60933