Research article - Peer-reviewed, 2020
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The CEP5 Peptide Promotes Abiotic Stress Tolerance, As Revealed by Quantitative Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis
Smith, Stephanie; Zhu, Shanshuo; Joos, Lisa; Roberts, Ianto; Nikonorova, Natalia; Vu, Lam Dai; Stes, Elisabeth; Cho, Hyunwoo; Larrieu, Antoine; Xuan, Wei; Goodall, Benjamin; van de Cotte, Brigitte; Waite, Jessic Marie; Rigal, Adeline; Harborough, Sigurd Ramans; Persiau, Geert; Vanneste, Steffen; Kirschner, Gwendolyn K.; Vandermarliere, Elien; Martens, Lennart; Stahl, Yvonne; Audenaert, Dominique; Friml, Jiri; Felix, Georg; Simon, Ruediger; Bennett, Malcolm J.; Bishopp, Anthony; De Jaeger, Geert; Ljung, Karin; Kepinski, Stefan; Robert, Stephanie; Nemhauser, Jennifer; Hwang, Ildoo; Gevaert, Kris; Beeckman, Tom; De Smet, IveShow less authors
Abstract
Peptides derived from non-functional precursors play important roles in various developmental processes, but also in (a)biotic stress signaling. Our (phospho)proteome-wide analyses of C-TERMINALLY ENCODED PEPTIDE 5 (CEP5)-mediated changes revealed an impact on abiotic stress-related processes. Drought has a dramatic impact on plant growth, development and reproduction, and the plant hormone auxin plays a role in drought responses. Our genetic, physiological, biochemical, and pharmacological results demonstrated that CEP5-mediated signaling is relevant for osmotic and drought stress tolerance in Arabidopsis, and that CEP5 specifically counteracts auxin effects. Specifically, we found that CEP5 signaling stabi-lizes AUX/IAA transcriptional repressors, suggesting the existence of a novel peptide-dependent control mechanism that tunes auxin signaling. These observations align with the recently described role of AUX/IAAs in stress tolerance and provide a novel role for CEP5 in osmotic and drought stress tolerance.Published in
Molecular and Cellular Proteomics2020, volume: 19, number: 8, pages: 1248-1262
Authors' information
Smith, Stephanie
University of Nottingham
Zhu, Shanshuo
Ghent University
Joos, Lisa
Ghent University
Roberts, Ianto
Ghent University
Nikonorova, Natalia
Ghent University
Vu, Lam Dai
Ghent University
Stes, Elisabeth
Ghent University
Cho, Hyunwoo
Ruprecht Karls University Heidelberg
Larrieu, Antoine
University of Leeds
Xuan, Wei
Ghent University
Goodall, Benjamin
University of Nottingham
van de Cotte, Brigitte
Ghent University
Waite, Jessic Marie
University of Washington
Rigal, Adeline
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Harborough, Sigurd Ramans
University of Leeds
Persiau, Geert
Ghent University
Vanneste, Steffen
Ghent University
Kirschner, Gwendolyn K.
Heinrich Heine University Dusseldorf
Vandermarliere, Elien
Ghent University
Martens, Lennart
Ghent University
Stahl, Yvonne
Heinrich Heine University Dusseldorf
Audenaert, Dominique
Ghent University
Friml, Jiri
Institute of Science and Technology - Austria
Felix, Georg
Eberhard Karls University of Tubingen
Simon, Ruediger
Heinrich Heine University Dusseldorf
Bennett, Malcolm J.
University of Nottingham
Bishopp, Anthony
University of Nottingham
De Jaeger, Geert
Ghent University
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Kepinski, Stefan
University of Leeds
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Nemhauser, Jennifer
University of Washington
Hwang, Ildoo
Pohang University of Science and Technology (POSTECH)
Gevaert, Kris
Ghent University
Beeckman, Tom
Ghent University
De Smet, Ive
University of Nottingham
UKÄ Subject classification
Biochemistry and Molecular Biology
Publication Identifiers
DOI: https://doi.org/10.1074/mcp.RA119.001826
URI (permanent link to this page)
https://res.slu.se/id/publ/106503