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Research article - Peer-reviewed, 2020

Geographical Distribution of Ljungan Virus in Small Mammals in Europe

Fevola, Cristina; Rossi, Chiara; Rosso, Fausta; Girardi, Matteo; Rosa, Roberto; Manica, Mattia; Delucchi, Luca; Rocchini, Duccio; Garzon Lopez, Carol X.; Arnoldi, Daniele; Bianchi, Alessandro; Buzan, Elena; Charbonnel, Nathalie; Collini, Margherita; Dureje, Ludovit; Ecke, Frauke; Ferrari, Nicola; Fischer, Stefan; Gillingham, Emma L.; Hornfeldt, Birger; Kazimirova, Maria; Konecny, Adam; Maas, Miriam; Magnusson, Magnus; Miller, Andrea L.; Niemimaa, Jukka; Nordstrom, Ake; Obiegala, Anna; Olsson, Gert E.; Pedrini, Paolo; Pialek, Jaroslav; Reusken, Chantal B.; Rizzolli, Franco; Romeo, Claudia; Silaghi, Cornelia; Sironen, Tarja; Stanko, Michal; Tagliapietra, Valentina; Ulrich, Rainer G.; Vapalahti, Olli; Voutilainen, Liina; Wauters, Lucas A.; Rizzoli, Annapaola; Vaheri, Antti; Jaaskelainen, Anne; Henttonen, Heikki; Hauffe, Heidi C.
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Abstract

Ljungan virus (LV), which belongs to the Parechovirus genus in the Picornaviridae family, was first isolated from bank voles (Myodes glareolus) in Sweden in 1998 and proposed as a zoonotic agent. To improve knowledge of the host association and geographical distribution of LV, tissues from 1685 animals belonging to multiple rodent and insectivore species from 12 European countries were screened for LV-RNA using reverse transcriptase (RT)-PCR. In addition, we investigated how the prevalence of LV-RNA in bank voles is associated with various intrinsic and extrinsic factors. We show that LV is widespread geographically, having been detected in at least one host species in nine European countries. Twelve out of 21 species screened were LV-RNA PCR positive, including, for the first time, the red vole (Myodes rutilus) and the root or tundra vole (Alexandromys formerly Microtus oeconomus), as well as in insectivores, including the bicolored white-toothed shrew (Crocidura leucodon) and the Valais shrew (Sorex antinorii). Results indicated that bank voles are the main rodent host for this virus (overall RT-PCR prevalence: 15.2%). Linear modeling of intrinsic and extrinsic factors that could impact LV prevalence showed a concave-down relationship between body mass and LV occurrence, so that subadults had the highest LV positivity, but LV in older animals was less prevalent. Also, LV prevalence was higher in autumn and lower in spring, and the amount of precipitation recorded during the 6 months preceding the trapping date was negatively correlated with the presence of the virus. Phylogenetic analysis on the 185 base pair species-specific sequence of the 5 ' untranslated region identified high genetic diversity (46.5%) between 80 haplotypes, although no geographical or host-specific patterns of diversity were detected.

Keywords

rodent vector; bank vole; reservoir host; Picornaviridae; cartogram; GLM

Published in

Vector-Borne and Zoonotic Diseases
2020, volume: 20, number: 9, pages: 692-702
Publisher: MARY ANN LIEBERT, INC

Authors' information

Fevola, Cristina
University of Helsinki
Rossi, Chiara
Fondazione Edmund Mach
Rosso, Fausta
Fondazione Edmund Mach
Girardi, Matteo
Fondazione Edmund Mach
Rosa, Roberto
University of Trento
Manica, Mattia
Fondazione Edmund Mach
Delucchi, Luca
Fondazione Edmund Mach
Rocchini, Duccio
University of Trento
Garzon Lopez, Carol X.
Fondazione Edmund Mach
Arnoldi, Daniele
Fondazione Edmund Mach
Bianchi, Alessandro
Its Zooprofilatt Sperimentale Lombardia and Emilia
Buzan, Elena
University of Primorska
Charbonnel, Nathalie
Universite de Montpellier
Collini, Margherita
University of Milan
Dureje, Ludovit
Czech Academy of Sciences
Swedish University of Agricultural Sciences, Department of Wildlife, Fish and Environmental Studies
Ferrari, Nicola
University of Milan
Fischer, Stefan
Friedrich Loeffler Institute
Gillingham, Emma L.
Cardiff University
Swedish University of Agricultural Sciences, Department of Wildlife, Fish and Environmental Studies
Kazimirova, Maria
Slovak Academy of Sciences
Konecny, Adam
Masaryk University Brno
Maas, Miriam
Netherlands National Institute for Public Health and the Environment
Swedish University of Agricultural Sciences, Department of Wildlife, Fish and Environmental Studies
Miller, Andrea
Swedish University of Agricultural Sciences, Department of Biomedical Science and Veterinary Public Health
Miller, Andrea
Norwegian Institute for Nature Research (NINA)
Niemimaa, Jukka
Natural Resources Institute Finland (Luke)
Swedish University of Agricultural Sciences, Department of Wildlife, Fish and Environmental Studies
Obiegala, Anna
University of Leipzig
Swedish University of Agricultural Sciences, Department of Wildlife, Fish and Environmental Studies
Pedrini, Paolo
MUSE Museo Sci
Pialek, Jaroslav
Czech Academy of Sciences
Reusken, Chantal B.
Erasmus University Rotterdam
Rizzolli, Franco
Fondazione Edmund Mach
Romeo, Claudia
University of Milan
Silaghi, Cornelia
University of Munich
Sironen, Tarja
University of Helsinki
Stanko, Michal
Slovak Academy of Sciences
Tagliapietra, Valentina
Fondazione Edmund Mach
Ulrich, Rainer G.
Friedrich Loeffler Institute
Vapalahti, Olli
University of Helsinki
Voutilainen, Liina
Natural Resources Institute Finland (Luke)
Wauters, Lucas A.
University of Insubria
Rizzoli, Annapaola
Fondazione Edmund Mach
Vaheri, Antti
University of Helsinki
Jaaskelainen, Anne
University of Helsinki
Henttonen, Heikki
Natural Resources Institute Finland (Luke)
Hauffe, Heidi C.
Fondazione Edmund Mach
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UKÄ Subject classification

Ecology
Microbiology

Publication Identifiers

DOI: https://doi.org/10.1089/vbz.2019.2542

URI (permanent link to this page)

https://res.slu.se/id/publ/106730