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Abstract

Introduction Since boar spermatozoa show a marked deterioration in sperm quality when cooled, insemination doses are usually stored at 16-18 degrees C. However, maintaining this temperature during transport of semen doses is challenging, particularly during the summer months. An alternative could be to store the doses at 4 degrees C if cold-shock to the sperm could be prevented. The objective of this study was to evaluate boar sperm quality and fertility in in vitro fertilization after storage in AndroStar Premium at 4 degrees C for 1 week. Methods Insemination doses (n = 9) in AndroStar Premium from a commercial boar semen collection station were transported to the laboratory at approximately 20 degrees C. At the laboratory, sperm quality evaluation and was preformed and each dose was split; half of each ejaculate was stored in a climate-controlled box at 16-18 degrees C, the other was slowly cooled to 4 degrees C. Both samples were stored for 1 week before further sperm quality evaluation and in vitro fertilization (IVF) were performed. Mean values were tested using generalized linear regression, with treatment and boar as fixed factors; p

Keywords

porcine; in vitro embryo production; semen storage; artificial insemination; long term boar semen storage

Published in

Frontiers in Veterinary Science
2024, volume: 11, article number: 1444550
Publisher: FRONTIERS MEDIA SA

SLU Authors

UKÄ Subject classification

Clinical Science

Publication identifier

  • DOI: https://doi.org/10.3389/fvets.2024.1444550

Permanent link to this page (URI)

https://res.slu.se/id/publ/132865