A Simplified Method for Gene Knockout and Direct Screening of Recombinant Clones for Application in Paenibacillus polymyxa

Abstract

Conclusions: We have created a simple system for targeted gene deletion in P. polymyxa E681. We propose that P. polymyxa isogenic mutants could be developed using this system of marker exchange mutagenesis. alpha- and beta-amylase genes provide a useful tool for direct recombinant screening in P. polymyxa.

Published in

PLoS ONE
2013, volume: 8, number: 6
Publisher: PUBLIC LIBRARY SCIENCE

SLU Authors

• seong-bin, Kim

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Timmusk, Salme

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

Associated SLU-program

AMR: Bacteria

Global goals (SDG)

SDG3 Good health and well-being

UKÄ Subject classification

Genetics

Publication identifier

• DOI: https://doi.org/10.1371/journal.pone.0068092

Permanent link to this page (URI)

https://res.slu.se/id/publ/54443