Structure and Morphology of Wheat Gluten Films: From Polymeric Protein Aggregates toward Superstructure Arrangements

Abstract

Evaluation of structure and morphology of extruded wheat gluten (WG) films showed WG protein assemblies elucidated on a range of length scales from nano (4.4 angstrom and 9 to 10 angstrom, up to 70 angstrom) to micro (10 mu m). The presence of NaOH in WG films induced a tetragonal structure with unit cell parameters, a = 51.85 angstrom and c = 40.65 angstrom, whereas NH(4)OH resulted in a bidimensional hexagonal close-packed (HCP) structure with a lattice parameter of 70 angstrom. In the WG films with NH(4)OH, a highly polymerized protein pattern with intimately mixed glutenins and gliadins bounded through SH/SS interchange reactions was found. A large content of beta-sheet structures was also found in these films, and the film structure was oriented in the extrusion direction. In conclusion, this study highlights complexities of the supramolecular structures and conformations of wheat gluten polymeric proteins in biofilms not previously reported for biobased materials.

Published in

Biomacromolecules
2011, volume: 12, number: 5, pages: 1438-1448
Publisher: AMER CHEMICAL SOC

SLU Authors

• Kuktaite, Ramune

  • Department of Biosystems and Technology, Swedish University of Agricultural Sciences
    

• Marttila, Salla

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

• Ignell, Rickard

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

• Johansson, Eva

  • Department of Biosystems and Technology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Genetics

Publication identifier

• DOI: https://doi.org/10.1021/bm200009h

Permanent link to this page (URI)

https://res.slu.se/id/publ/56977