Time-series metatranscriptomes reveal conserved patterns between phototrophic and heterotrophic microbes in diverse freshwater systems

Abstract

Microbial communities form the base of food webs in freshwater ecosystems, yet the interactions within these diverse assemblages are poorly understood. Based on evidence showing that primary production and respiration follow diurnal trends in lakes, we hypothesized that gene expression in freshwater microbes would have similar diel cycles. We used three 2-d time series of metatranscriptomes to test this hypothesis in a eutrophic lake, an oligotrophic lake, and a humic lake. We identified prominent diel cycles in all three lakes, particularly in genes related to photosynthesis, sugar transport, and carbon fixation. The maximal time of expression for sugar transport genes tended to trail that of photosynthesis genes by several hours, indicating possible metabolic exchange between co-occurring microbial lineages. These results provide an initial step in understanding sophisticated multispecies transcriptional organization within freshwater microbial communities.

Published in

Limnology and Oceanography
2020, Volume: 65, number: Supplement 1, pages: S101-S112 Publisher: WILEY

SLU Authors

• Bertilsson, Stefan

  • Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Oceanography, Hydrology, Water Resources
Microbiology


Publication identifier

DOI: https://doi.org/10.1002/lno.11306

Permanent link to this page (URI)

https://res.slu.se/id/publ/101651