Genome constellations of rotavirus a isolated from avian species in Brazil, 2008-2015

Abstract

Rotaviruses are members of the family Reoviridae and are a common cause of acute diarrhea in many mammalian and avian species. They are non-enveloped icosahedral particles and their genome comprises 11 segments of double-stranded RNA, which encodes six structural proteins (VP1-4, VP6-7) and six nonstructural proteins (NSP1-6). Genotypes are defined based upon the diversity found in these genes and viral characterization plays a central role on epidemiological studies and prevention. Here we investigate the distribution of Brazilian RVAs genotypes in 8 chicken samples collected between 2008 and 2015 from different regions by RT-PCR, partial (Sanger) nucleotide sequencing and phylogenetic analysis from all rotavirus genes. Although the identified genotypes were typical from avian host species, when analyzed together, they form novel genetic constellations: G19-P[31]-I11-R6-C6-M7-A16-N6-T8-E10-H8 and G19-P[31]-I4-R4-C4-M4-A16-N4-T4-E4-H4. This study highlights that avian rotaviruses are widespread among commercial farms in Brazil, and the co-circulation of at least two different genomic constellations indicates that may present a way bigger genetic variability, that can be increased by the possible transmission events from other birds, lack of specific preventive measures, as well as the different viral evolution mechanisms.

Keywords

Rotavirus; Avian; Diversity; Genotypes; Sequence analysis; Phylogeny

Published in

Brazilian Journal of Microbiology
2020, Volume: 51, number: 3, pages: 1363-1375 Publisher: SPRINGER

SLU Authors

• Berg, Mikael

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Microbiology


More information

Correction in: Brazilian Journal of Microbiology, 2020, DOI: 10.1007/s42770-020-00407-w


Publication identifier

DOI: https://doi.org/10.1007/s42770-020-00259-4

Permanent link to this page (URI)

https://res.slu.se/id/publ/105685