Abstract

Mutation breeding based on various chemical and physical mutagens induces and disrupts non-target loci. Hence, large populations were required for visual screening, but desired plants were rare and it was a further laborious task to identify desirable mutants. Generated mutant had high defect due to non-targeted mutation, with poor agronomic performance. Mutation techniques were augmented by targeted induced local lesions in genome (TILLING) facilitating the selection of desirable germplasm. On the other hand, gene editing through CRISPR/Cas9 allows knocking down genes for site-directed mutation. This handy technique has been exploited for the modification of fatty acid profile. High oleic acid genetic stocks were obtained in a broad range of crops. Moreover, genes involved in the accumulation of undesirable seed components such as starch, polysaccharide, and flavors were knocked down to enhance seed quality, which helps to improve oil contents and reduces the anti-nutritional component.

Keywords

Gene editing; genome; induced mutation; oil contents; sunflower; >

Published in

GM Crops & Food
2023, Volume: 14, number: 1, pages: 1-12
Publisher: TAYLOR AND FRANCIS AS

SLU Authors

• Ortiz Rios, Rodomiro Octavio

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

Sustainable Development Goals

SDG13 Take urgent action to combat climate change and its impacts
SDG2 End hunger, achieve food security and improved nutrition and promote sustainable agriculture
SDG1 End poverty in all its forms everywhere


UKÄ Subject classification

Plant Biotechnology
Agricultural Science


Publication identifier

DOI: https://doi.org/10.1080/21645698.2023.2243041

Permanent link to this page (URI)

https://res.slu.se/id/publ/123055