Abstract

RNA interference (RNAi) is a conserved cellular defense mechanism mediated by double-stranded RNA (dsRNA) that can regulate gene expression through targeted destruction of mRNAs (messenger RNAs). Recent studies have shown that spraying dsRNAs or small RNAs (sRNAs) that target essential genes of pathogens on plant surfaces can confer protection against pests and pathogens. Also called spray-induced gene silencing (SIGS), this strategy can be used for disease control and for transient gene silencing to study the function of genes in plant−pathogen interactions. Furthermore, as sRNAs can move locally, systemically, and cross-kingdom during plant−microbe interactions, SIGS allows quick detection and characterization of gene functions in pathogens and plants.

Keywords

Gene silencing; Oomycetes; Potato; RNAi; SIGS

Published in

Methods in Molecular Biology
2022, Volume: 2536, number: 2536, pages: 459-474 Title: Plant Pathology : Method and Protocols
ISBN: 978-1-0716-2516-3, eISBN: 978-1-0716-2517-0
Publisher: Springer

SLU Authors

• Sundararajan, Poorva

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

• Kalyandurg, Pruthvi Balachandra

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

• Chawade, Aakash

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

• Vetukuri, Ramesh

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Genetics


Publication identifier

DOI: https://doi.org/10.1007/978-1-0716-2517-0_27

Permanent link to this page (URI)

https://res.slu.se/id/publ/129739