Moschou, Panagiotis Nikolaou
- Department of Plant Biology, Swedish University of Agricultural Sciences
Research article2018Peer reviewed
Determination of di-/Polyamine Oxidase Activity in Plants by an In-Gel Spermidine Oxidation Assay
Moschou, Panagiotis Nikolaou
Abstract
Diamine and polyamine catabolism controls plant development, resistance to pathogens and stress responses. Diamine and polyamine oxidases control the catabolism of diamines and polyamines, respectively. Two major routes of di-/polyamine catabolism exist: the terminal and the interconverting. The in vitro activity of each route is assayed by the colorimetric or chemiluminescent determination of hydrogen peroxide produced by oxidation of di-/polyamine substrates. However, these assays fail to estimate activity of individual di-/polyamine oxidase isoenzymes. Herein, I describe an assay for the simultaneous in-gel determination of terminal and interconverting di-/polyamine oxidase isoenzyme activities.
Published in
Methods in Molecular Biology
2018, volume: 1694, pages: 141-147
SLU Authors
Associated SLU-program
SLU Plant Protection Network
UKÄ Subject classification
Agricultural Science
Publication identifier
- DOI: https://doi.org/10.1007/978-1-4939-7398-9_14
Permanent link to this page (URI)
https://res.slu.se/id/publ/111043