Stored Stallion Sperm Quality Depends on Sperm Preparation Method in INRA82 or INRA96

Papin (nee Richter), Johanna; Stuhtmann, Gesa; Martinsson, Gunilla; Sieme, Harald; Lundeheim, Nils; Ntallaris, Theodoros; Morrell, Jane M.

doi:10.1016/j.jevs.2020.103367

Research article2021Peer reviewedOpen access

Stored Stallion Sperm Quality Depends on Sperm Preparation Method in INRA82 or INRA96

Papin (nee Richter), Johanna; Stuhtmann, Gesa; Martinsson, Gunilla; Sieme, Harald; Lundeheim, Nils; Ntallaris, Theodoros; Morrell, Jane M.

Abstract

Removal of seminal plasma facilitates stallion sperm survival during storage, but washing may damage sperm chromatin. Therefore, sperm quality was compared in samples following single-layer centrifugation (SLC) or sperm washing and controls (extension only) in two extenders, INRA82 and INRA96. Ejaculates from six stallions were split among six treatments: SLC, sperm washing, and controls, in INRA82 and INRA96. Sperm motility and acrosome status were evaluated at 0, 24, 48, 72 and 96 hours; morphology at 0, 24, 48, 72 hours and chromatin integrity at 0 and 96 hours, with storage at 6 degrees C. Sperm samples in INRA96 had better motility, acrosome status, and normal morphology than samples in INRA82. The SLC samples had higher motility and fewer reacted acrosomes than controls, and lower fragmented chromatin than washed samples. Fewer spermatozoa with tail defects were observed after SLC than after sperm washing; spermatozoa washed in INRA82 had fewer tail defects than those washed in INRA96. In conclusion, sperm quality (except for morphology) was better in INRA96 than in INRA82 and was better in SLC samples than in washed samples or controls. The SLC method is a useful adjunct to stallion sperm preparation, especially for storage before artificial insemination. (C) 2020 The Author(s). Published by Elsevier Inc.

Keywords

Colloid centrifugation; Stallion semen; Sperm motility; Chromatin integrity; Acrosome status

Published in

Journal of Equine Veterinary Science
2021, volume: 98, article number: 103367

SLU Authors

Lundeheim, Nils
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Ntallaris, Theodoros
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
Morrell, Jane
- Department of Clinical Sciences, Swedish University of Agricultural Sciences

UKÄ Subject classification

Clinical Science

Publication identifier

DOI: https://doi.org/10.1016/j.jevs.2020.103367

Permanent link to this page (URI)

https://res.slu.se/id/publ/111174