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Research article2021Peer reviewedOpen access

Single-Shot Vaccines against Bovine Respiratory Syncytial Virus (BRSV): Comparative Evaluation of Long-Term Protection after Immunization in the Presence of BRSV-Specific Maternal Antibodies

Valarcher, Jean Francois; Hagglund, Sara; Naslund, Katarina; Jouneau, Luc; Malmstrom, Ester; Boulesteix, Olivier; Pinard, Anne; Leguere, Dany; Deslis, Alain; Gauthier, David; Dubuquoy, Catherine; Pietralunga, Vincent; Remot, Aude; Falk, Alexander; Shevchenko, Ganna; Bergstrom Lind, Sara; Von Bromssen, Claudia; Vargmar, Karin; Zhang, Baoshan; Kwong, Peter D.;
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Abstract

The induction of long-lasting clinical and virological protection is needed for a successful vaccination program against the bovine respiratory syncytial virus (BRSV). In this study, calves with BRSV-specific maternally derived antibodies were vaccinated once, either with (i) a BRSV pre-fusion protein (PreF) and Montanide(TM) ISA61 VG (ISA61, n = 6), (ii) BRSV lacking the SH gene (Delta SHrBRSV, n = 6), (iii) a commercial vaccine (CV, n = 6), or were injected with ISA61 alone (n = 6). All calves were challenged with BRSV 92 days later and were euthanized 13 days post-infection. Based on clinical, pathological, and proteomic data, all vaccines appeared safe. Compared to the controls, PreF induced the most significant clinical and virological protection post-challenge, followed by Delta SHrBRSV and CV, whereas the protection of PreF-vaccinated calves was correlated with BRSV-specific serum immunoglobulin (Ig)G antibody responses 84 days post-vaccination, and the IgG antibody titers of Delta SHrBRSV- and CV-vaccinated calves did not differ from the controls on this day. Nevertheless, strong anamnestic BRSV- and PreF-specific IgG responses occurred in calves vaccinated with either of the vaccines, following a BRSV challenge. In conclusion, PreF and Delta SHrBRSV are two efficient one-shot candidate vaccines. By inducing a protection for at least three months, they could potentially improve the control of BRSV in calves.

Keywords

respiratory syncytial virus; bovine; neonatal; subunit vaccine; pre-fusion; live-attenuated vaccine; deleted SHrBRSV; one-shot; duration of protection; correlate of protection

Published in

Vaccines
2021, volume: 9, number: 3, article number: 236
Publisher: MDPI

SLU Authors

UKÄ Subject classification

Clinical Science

Publication identifier

  • DOI: https://doi.org/10.3390/vaccines9030236

Permanent link to this page (URI)

https://res.slu.se/id/publ/111627