A fast, nondestructive method for the detection of disease-related lesions and wounded leaves

Abstract

Trypan blue staining is a classic way of visualizing leaf disease and wound responses in plants, but it involves working with toxic chemicals and is time-consuming (2-3 days). Here, the investigators established near-infrared scanning with standard lab equipment as a fast and nondestructive method for the analysis of leaf injuries compared with trypan blue staining. Pathogen-inoculated and wounded leaves from potato, tomato, spinach, strawberry, and arabidopsis plants were used for proof of concept. The results showed that this newly developed protocol with near-infrared scanning gave the same results as trypan blue staining. Furthermore, a macro in FIJI was made to quantify the leaf damage. The new protocol was time-efficient, nondestructive, chemical-free and may be used for high-throughput studies.

Keywords

image processing; leaf damage; lesions; phenotyping; plant disease; potato; Phytophthora

Published in

Biotechniques
2021, volume: 71, number: 2
Publisher: FUTURE SCI LTD

SLU Authors

• Zahid, Muhammad Awais

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

• Araujo Sandroni, Murilo

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

• Vetukuri, Ramesh

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

• Andreasson, Erik

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Agricultural Science

Publication identifier

• DOI: https://doi.org/10.2144/btn-2021-0045

Permanent link to this page (URI)

https://res.slu.se/id/publ/113434