Circum-Arctic distribution of chemical anti-herbivore compounds suggests biome-wide trade-off in defence strategies in Arctic shrubs

Abstract

Spatial variation in plant chemical defence towards herbivores can help us understand variation in herbivore top-down control of shrubs in the Arctic and possibly also shrub responses to global warming. Less defended, non-resinous shrubs could be more influenced by herbivores than more defended, resinous shrubs. However, sparse field measurements limit our current understanding of how much of the circum-Arctic variation in defence compounds is explained by taxa or defence functional groups (resinous/non-resinous). We measured circum-Arctic chemical defence and leaf digestibility in resinous (Betula glandulosa, B. nana ssp. exilis) and non-resinous (B. nana ssp. nana, B. pumila) shrub birches to see how they vary among and within taxa and functional groups. Using liquid chromatography-mass spectrometry (LC-MS) metabolomic analyses and in vitro leaf digestibility via incubation in cattle rumen fluid, we analysed defence composition and leaf digestibility in 128 samples from 44 tundra locations. We found biogeographical patterns in anti-herbivore defence where mean leaf triterpene concentrations and twig resin gland density were greater in resinous taxa and mean concentrations of condensing tannins were greater in non-resinous taxa. This indicates a biome-wide trade-off between triterpene- or tannin-dominated defences. However, we also found variations in chemical defence composition and resin gland density both within and among functional groups (resinous/non-resinous) and taxa, suggesting these categorisations only partly predict chemical herbivore defence. Complex tannins were the only defence compounds negatively related to in vitro digestibility, identifying this previously neglected tannin group as having a potential key role in birch anti-herbivore defence. We conclude that circum-Arctic variation in birch anti-herbivore defence can be partly derived from biogeographical distributions of birch taxa, although our detailed mapping of plant defence provides more information on this variation and can be used for better predictions of herbivore effects on Arctic vegetation.

Keywords

Arctic; Betula; birch; herbivory; metabolomics; plant chemical defence; shrubs; tundra

Published in

Ecography
2022, volume: 2022, number: 11, article number: e06166

SLU Authors

• Abreu, Ilka

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    
  • University of Göttingen

• Moritz, Thomas

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

• Sundqvist, Maja

  • Department of Forest Ecology and Management, Swedish University of Agricultural Sciences
    

• Skarin, Anna

  • Department of Applied Animal Science and Welfare, Swedish University of Agricultural Sciences
    

Associated SLU-program

SLU Plant Protection Network

Global goals (SDG)

SDG13 Climate action

UKÄ Subject classification

Ecology

Publication identifier

• DOI: https://doi.org/10.1111/ecog.06166

Permanent link to this page (URI)

https://res.slu.se/id/publ/118664