Physiology-informed toxicokinetic model for the zebrafish embryo test developed for bisphenols

Abstract

Zebrafish embryos (ZFE) is a widely used model organism, employed in various research fields including toxicology to assess e.g., developmental toxicity and endocrine disruption. Variation in effects between chemicals are difficult to compare using nominal dose as toxicokinetic properties may vary. Toxicokinetic (TK) modeling is a means to estimate internal exposure concentration or dose at target and to enable extrapolation between experimental conditions and species, thereby improving hazard assessment of potential pollutants. In this study we advance currently existing TK models for ZFE with physiological ZFE parameters and novel experimental bisphenol data, a class of chemicals with suspected endocrine activity. We developed a five-compartment model consisting of water, plastic, chorion, yolk sack and embryo in which surface area and volume changes as well as the processes of biotransformation and blood circulation influence mass fluxes. For model training and validation, we measured internal concentrations in ZFE exposed individually to BPA, bisphenol AF (BPAF) and Z (BPZ).

Keywords

Embryo; PBTK; Zebrafish; Bisphenols; Endocrine disruptors

Published in

Chemosphere
2023, volume: 345, article number: 140399

SLU Authors

• Vogs, Carolina

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    
  • Karolinska Institute

• Örn, Stefan

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Pharmacology and Toxicology
Medical Bioscience

Publication identifier

• DOI: https://doi.org/10.1016/j.chemosphere.2023.140399

Permanent link to this page (URI)

https://res.slu.se/id/publ/127217