Kindberg, Jonas
- Department of Wildlife, Fish and Environmental Studies, Swedish University of Agricultural Sciences
- Norwegian Institute for Nature Research (NINA)
Research article2025Peer reviewedOpen access
Reduced ATP turnover during hibernation in relaxed skeletal muscle
De Napoli, Cosimo; Schmidt, Luisa; Montesel, Mauro; Cussonneau, Laura; Sanniti, Samuele; Marcucci, Lorenzo; Germinario, Elena; Kindberg, Jonas; Evans, Alina Lynn; Gauquelin-Koch, Guillemette; Narici, Marco; Bertile, Fabrice; Lefai, Etienne; Krueger, Marcus; Nogara, Leonardo; Blaauw, Bert
Abstract
Hibernating brown bears, due to a drastic reduction in metabolic rate, show only moderate muscle wasting. Here, we evaluate if ATPase activity of resting skeletal muscle myosin can contribute to this energy sparing. By analyzing single muscle fibers taken from the same bears, either during hibernation or in summer, we find that fibers from hibernating bears have a mild decline in force production and a significant reduction in ATPase activity. Single fiber proteomics, western blotting, and immunohistochemical analyses reveal major remodeling of the mitochondrial proteome during hibernation. Furthermore, using bioinformatical approaches and western blotting we find that phosphorylated myosin light chain, a known stimulator of basal myosin ATPase activity, is decreased in hibernating and disused muscles. These results suggest that skeletal muscle limits energy loss by reducing myosin ATPase activity, indicating a possible role for myosin ATPase activity modulation in multiple muscle wasting conditions.
Published in
Nature Communications
2025, volume: 16, number: 1, article number: 80
Publisher: NATURE PORTFOLIO
SLU Authors
UKÄ Subject classification
Physiology
Zoology
Publication identifier
- DOI: https://doi.org/10.1038/s41467-024-55565-4
Permanent link to this page (URI)
https://res.slu.se/id/publ/140198