Increased Recovery of Green Doubled Haploid Plants from Barley Anther Culture

Abstract

Albinism is a major limitation in the production of doubled haploid plants in cereals. As this trait is partly genetically controlled, the culture method has to be adapted to the plant genotype to reduce albinism. We have improved green plant recovery in the Northern barley (Hordeum vulgare L.) spring cultivar Mitja from 6 to 42% by altering the culture method and composition of the culture media. In a new three-step protocol, the addition of casein hydrolysate to an intermediate regeneration medium gave the highest number of green plants. The shoot and root growth medium used for germinated embryos was superior to the standard media reported in the literature for root formation and shoot growth. Our modified method has merit for testing with other recalcitrant genotypes in anther and isolated microspore cultures.

Published in

Crop Science
2015, volume: 55, number: 6, pages: 2806-2812
Publisher: CROP SCIENCE SOC AMER

SLU Authors

• Sriskandarajah, Sridevy

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Sameri, Mohammad

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Lerceteau-Köhler, Estelle

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Westerbergh, Anna

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Plant Biotechnology

Publication identifier

• DOI: https://doi.org/10.2135/cropsci2015.04.0245

Permanent link to this page (URI)

https://res.slu.se/id/publ/72398