Ranade, Sonali
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
Research article2015Peer reviewedOpen access
Fungal Infection Increases the Rate of Somatic Mutation in Scots Pine (Pinus sylvestris L.)
Ranade, Sonali Sachin; Ganea, Laura Stefana; Razzak, Abdur R.; Garcia Gil, Rosario
Abstract
Somatic mutations are transmitted during mitosis in developing somatic tissue. Somatic cells bearing the mutations can develop into reproductive (germ) cells and the somatic mutations are then passed on to the next generation of plants. Somatic mutations are a source of variation essential to evolve new defense strategies and adapt to the environment. Stem rust disease in Scots pine has a negative effect on wood quality, and thus adversely affects the economy. It is caused by the 2 most destructive fungal species in Scandinavia: Peridermium pini and Cronartium flaccidum. We studied nuclear genome stability in Scots pine under biotic stress (fungus-infected, 22 trees) compared to a control population (plantation, 20 trees). Stability was assessed as accumulation of new somatic mutations in 10 microsatellite loci selected for genotyping. Microsatellites are widely used as molecular markers in population genetics studies of plants, and are particularly used for detection of somatic mutations as their rate of mutation is of a much higher magnitude when compared with other DNA markers. We report double the rate of somatic mutation per locus in the fungus-infected trees (4.8 x 10(-3) mutations per locus), as compared to the controls (2.0 x 10(-3) mutations per locus) when individual samples were analyzed at 10 different microsatellite markers. Pearson's chi-squared test indicated a significant effect of the fungal infection which increased the number of mutations in the fungus-infected trees (chi(2) = 12.9883, df = 1, P = 0.0003134).
Keywords
abiotic stress; microsatellite; simple sequence repeats; Scots pine; Somatic mutation
Published in
Journal of Heredity
2015, volume: 106, number: 4, pages: 386-394
Publisher: OXFORD UNIV PRESS INC
SLU Authors
Ganea, Laura Stefana
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
Garcia Gil, Rosario
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
Associated SLU-program
SLU Plant Protection Network
UKÄ Subject classification
Genetics
Forest Science
Publication identifier
- DOI: https://doi.org/10.1093/jhered/esv017
Permanent link to this page (URI)
https://res.slu.se/id/publ/75604