The constitutive expression and induction of three β-1,3-glucanases by bird cherry-oat aphid in relation to aphid resistance in 15 barley breeding lines

Abstract

In this study, 15 closely related barley genotypes were analyzed for the abundance of three beta-1,3-glucanase transcripts immediately before and during infestation by the bird cherry-oat aphid (Rhopalosiphum padi L.). The barley lines are doubled haploid lines in backcross (BC) generations BC1 and BC2 from a cross between cultivar Lina and a wild barley accession. Previously, they have been characterized as susceptible (S) or resistant (R) to R. padi based on their ability to support nymphal growth. Here we also tested whether resistance was manifested as reduced aphid settling on the plants. Indeed, aphid numbers were lower on R than on S lines in all cases where there were significant differences between R and S lines. The choice of beta-1,3-glucanase sequences is based on earlier results comparing two S and two R genotypes, suggesting that at least two of the three studied sequences are susceptibility factors. The comparisons of transcript abundance in plants with aphids showed for two of the beta-1,3-glucanase sequences that there were several cases where an S genotype had significantly higher abundance than an R genotype, and in no case did an R line have significantly higher abundance than an S line. Thus, there was some support for the idea that beta-1,3-glucanase sequences are susceptibility factors in the interaction between barley and R. padi.

Keywords

Aphid resistance; Aphid susceptibility; Rhopalosiphum padi; Hordeum vulgare

Published in

Arthropod-Plant Interactions
2016, volume: 10, number: 2, pages: 101-111

SLU Authors

• Mehrabi, Sara

  • Stockholm University

• Åhman, Inger

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

Associated SLU-program

SLU Plant Protection Network

UKÄ Subject classification

Genetics and Breeding
Plant Biotechnology

Publication identifier

• DOI: https://doi.org/10.1007/s11829-016-9415-2

Permanent link to this page (URI)

https://res.slu.se/id/publ/79626